9L/lacZ (大鼠胶质肉瘤细胞) (种属鉴定正确)

名称

9L/lacZ (大鼠胶质肉瘤细胞) (种属鉴定正确)

别称

9L/LacZ

种属

大鼠

年龄（性别）

雄性

组织来源

脑，胶质细胞

生长特性

贴壁细胞

细胞形态

成纤维细胞样

背景描述

The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed on tissue sections by histochemical stain, and single tumor cells can be identified. Lymphocytes and other responding cells can be identified by double labeling with antibodies on the same slide. The contrast between stained cells and background facilitates image analysis. The beta-gal expression is very stable, but cells may need to be re-cloned after months of growth in culture.

生物安全等级

1

生长培养基

推荐传代比例

1:4-1:8

推荐换液频率

2~3次/周

冻存条件

冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮

培养条件

气相：空气，95%；CO2，5%温度：37℃

致瘤性

Yes, forms tumors in the brains of CD Fischer 344 rats

基因表达情况

beta galactosidase (beta-gal)

保藏机构

ATCC; CRL-2200