M-07e（人巨细胞白血病细胞）(STR鉴定正确)

名称

M-07e（人巨细胞白血病细胞）(STR鉴定正确)

别称

M-07E; M-O7e; M07-e; M07e; Mo7e; MO7e; M07E; MO7E

种属

人类

年龄（性别）

女性，6个月

组织来源

外周血

生长特性

悬浮细胞

细胞形态

淋巴母细胞样

背景描述

Established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-beta, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells. Exome and RNA sequence data are available

生物安全等级

1

生长培养基

RPMI-1640＋10% FBS＋8ng/mL GM-CSF+1% P/S

推荐传代比例

5×10^5-1×10^6cells/mL

推荐换液频率

2~3次/周

倍增时间

~40小时

冻存条件

冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮

培养条件

气相：空气，95%；CO2，5%温度：37℃

抗原表达情况

CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR -

注意事项

该细胞为悬浮细胞，请注意离心收集细胞悬液；请勿直接倒掉细胞培养液。

保藏机构

DSMZ; ACC-104