Predicted molecular mass
6.2 kDa
Specification:
Appearance White powder, Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions,
visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Measured in a cell proliferation assay using NIH3T3 mouse embryonic
fibroblast Cells. The ED50 for this effect ≤0.3 ng/mL.
Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol
and 0.01% Tween 80, pH 7.4 Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice.
Storage & Stability 36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
SDS-PAGE
Recombinant Human EGF SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with SDS-PAGE.
SEC-HPLC
